Part:BBa_J435605

pOSIP-KO (KanR, 186)

BBF10K_000492

One-Step Cloning and Chromosomal Integration of DNA

Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.

antibiotic: Kanamycin

https://freegenes.github.io/genes/BBF10K_000492.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found in sequence at 1125
Illegal EcoRI site found at 1
Illegal XbaI site found at 28
Illegal XbaI site found at 1397
Illegal XbaI site found at 4823
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
Illegal NheI site found at 3312
Illegal NheI site found at 5166
Illegal SpeI site found at 1126
Illegal PstI site found at 1140
Illegal NotI site found at 1133
Illegal NotI site found at 4358
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
Illegal BglII site found at 469
Illegal BamHI site found at 22
Illegal XhoI site found at 1152
Illegal XhoI site found at 5081
23
INCOMPATIBLE WITH RFC[23]
Illegal suffix found in sequence at 1126
Illegal EcoRI site found at 1
Illegal XbaI site found at 28
Illegal XbaI site found at 1397
Illegal XbaI site found at 4823
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
Illegal XbaI site found at 28
Illegal XbaI site found at 1397
Illegal XbaI site found at 4823
Illegal SpeI site found at 1126
Illegal PstI site found at 1140
Illegal AgeI site found at 1343
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 357